Coupling Constants in Proteins
Hydrogen Bond Experiments
S3CTMeasurement of one-bond coupling constants
Sequence= (2JSI)-1; The gradients -G and G are omitted for the first few t1 increments until the delay is large enough to accomodate them. and are cycled to create four data sets:
Appropriate linear combinations yield the pairs of echo/antiecho data sets for the two subspectra:
Expansion from a 2D S3CT spectrum of 1mM 15N-labeled CI2 in bicelle solution at 37°C. The two subspectra (black and red) are superimposed. Values for 1JNH + 1DNH (upper) and 1JNH (lower) are given next to the peaks.
Document: S3CT (index.shtml)
Last modified: 2005-12-19