Edited HMBC for Bruker

Pulse program

• Multiplicity edited HMBC with third order low-pass J-filter:
  edhmbc

AU-programs for processing

• General split and combination of interleaved 2D-data:
  splitcomb
• General split of interleaved 2D-data:
  splitil
• Splitting and adding several edhmbc-spectra (as in broad-band hmbc, needs the AU-programs splitcomb, madd2d and mxfbxf2m):
  p_bbhmbc
• Transforming several spectra:
  mxfbxf2m
• Transforming several spectra:
  mxfb
• Adding several spectra (as in broad-band hmbc):
  madd2d

Procedure for pulseprogram edhmbc

1. Start with a HMBC parameterset rpar HMBCGP all
2. Change pulse program to edhmbc pulprog edhmbc
3. Set FnMODE to Echo-Antiecho
4. Set up the acquisition parameters ased
5. Set cnst6 to 125 Hz and cnst8 to 165 Hz. The preparation delay, d6 can initially be set to 65 ms.
6. • For a standard HMBC-experiment:
     set the ZGOPTNS to -DSUB and L31 to 0 ZGOPTNS -DSUB; l31 0
   • For a up-down HMBC-experiment:
     set the ZGOPTNS to -DSUB and L31 to 1 ZGOPTNS -DSUB; l31 1
   • For an edited HMBC-experiment (i.e.,standard and up-down experiments acquierd in an interleaved mode):
     leave ZGOPTNS empty
7. Set the number of scans to ~ 4× that of a decent HSQC on the same sample. Keep the same receiver gain.

Processing of interleaved data

1. Split and combine the dataset to two new datasets: splitcomb 2
2. Change to the first of the two datasets: re <expno×100+1>
3. Calculate the 'mixed mode' magnitude mode 1 phc0 90; 1 phmod pk; 2 phmod no; xfb; xf2m
4. Process the second dataset (<expno×100+2>) the same way.
5. The second dataset contains signals from CH and CH₃-groups while the first dataset contains signals from CH₂-groups.