Improved Edited HMBC for Bruker

Pulse program

• Multiplicity edited HMBC with third order low-pass J-filter:
  edhmbc.2

AU-programs for processing

• Simple processing macro that splits, combines and processes the spectra
  edhmbc2proc

Procedure for pulseprogram edhmbc

1. Start with a HMBC parameterset rpar HMBCGP all
2. Change pulse program to edhmbc pulprog edhmbc
3. Set FnMODE to Echo-Antiecho
4. Set up the acquisition parameters ased
5. Set cnst6 to 125 Hz and cnst8 to 165 Hz. The preparation delay, d6 can initially be set to 65 ms.
6. Set TD for F1 to 4 times the desired digital resolution in the final spectra.
7. Set the number of scans to ~ 4× that of a decent HSQC on the same sample. (The minimum number of scans is 4.) Keep the same receiver gain.