TROSY

Ref.:
T.Schulte-Herbrüggen, and O.W.Sørensen J.Magn.Reson. 144, 123-128 (2000). (Clean TROSY: compensation for relaxation-induced artefacts)

M.H.Lerche, A.Meissner, F.M.Poulsen, and O.W.Sørensen J.Magn.Reson. 140, 259-263 (1999). (Pulse Sequences for Measurement of One-Bond 15N-1H Coupling Constants in the Protein Backbone)

A.Meissner, T.Schulte-Herbrueggen, J.Briand, and O.W.Sørensen Mol.Phys. 95, 1137-1142 (1998). (Double spin-state-selective coherence transfer. Application for two-dimensional selection of multiplet components with long transverse relaxation times)

(clean) TROSY pulse sequences without and with decoupling during t2.
Delays:  = S = (2JNH)-1 and I = (2JNH)-1 - I.
Phases for Varian Unity Inova:   = -y; I = +y, S = +y - S (echo), I = -y, S = -y - S (antiecho).
Phases for Bruker DRX:   = y; I = -y, S = -y - S (echo), I = +y, S = +y - S (antiecho).
The uncompensated TROSY experiment employs S = I = 0.
Gradient ratio:  -7.0(G1), 3.0(G2), 2.0(G3) for echo;  -8.0(G1), 2.0(G2), 3.0(G3) for antiecho.

For water flip back it is crucial to set the transmitter frequency of the protons right on the water resonance.

Varian:

Sequence 1 : Set parameter dm2='nnn'
Sequence 2 : Set parameter dm2='nny'

For clean-TROSY set clean = 'y'
cdel: J evolution during first CT element (in degrees), 90 = full delay
cphas: phase shift in second CT element (in degrees), 0 = no phase shift

To generate a selective water pulse shape, copy water_sc.inp to your $HOME/vnmrsys/shapelib directory, adjust ref_pwr and ref_pw90 according to your proton pulse calibration and type the command: Pbox -f water_sc.inp

An output like
>>> Set pulse width to 1.0000 ms <<<
>>> Set pulse power to 21 dB <<<
appears.

Remember to change the parameters pwater (pulse width in us) and pwaterlvl (pulse power).

Program tested on: Varian Unity Inova, Vnmr6.1B, Pbox6.1C, Solaris7

Content
Pulse sequence:  trosy.c
Parameters:      trosy.par
Pbox input file: water_sc.inp

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Bruker:

Sequence 1 : trosy
Sequence 2 : trosy_d2

For clean TROSY set "d4=1s*60/(cnst2*360)" and ph18 = (360) {86 86}^180 (values may be adjusted according to sample properties)
for non compensated TROSY set  "d4=1s*90/(cnst2*360)" and ph18 = (360) {90 90}^180
(ph18 is omitted in sequence 2)

Program tested on: Bruker DRX600, Xwinnmr2.6 patchlevel 4, Irix6.3

Content
Pulse sequence:   trosy
Gradient program: clean_trosy.r

Pulse sequence:   trosy_d2
Gradient program: clean_trosy_d2.r

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Document: TROSY (index.shtml)
Last modified: 2005-12-19